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ebv peptide mix  (Miltenyi Biotec)


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    Miltenyi Biotec ebv peptide mix
    CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent <t>donors.</t> <t>PBMCs</t> from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with <t>EBV</t> peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.
    Ebv Peptide Mix, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ebv+peptide+mix/MACS+GMP+PepTivator+EBV+Select/pmc08579755-306-64-84
    Average 93 stars, based on 4 article reviews
    ebv peptide mix - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity"

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity

    Journal: Journal of Virology

    doi: 10.1128/JVI.02002-20

    CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent donors. PBMCs from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with EBV peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.
    Figure Legend Snippet: CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent donors. PBMCs from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with EBV peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.

    Techniques Used: Ex Vivo, Derivative Assay, Negative Control, Positive Control

    Related Articles

    Incubation:

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity
    Article Snippet: .. The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec). .. After the incubation with these peptides, the cells were washed once with RPMI and costimulated using purified anti-human CD28 antibody (1 μg/ml) (clone L293; BD Biosciences).

    Virus:

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity
    Article Snippet: .. The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec). .. After the incubation with these peptides, the cells were washed once with RPMI and costimulated using purified anti-human CD28 antibody (1 μg/ml) (clone L293; BD Biosciences).

    Magnetic Cell Separation:

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity
    Article Snippet: .. The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec). .. After the incubation with these peptides, the cells were washed once with RPMI and costimulated using purified anti-human CD28 antibody (1 μg/ml) (clone L293; BD Biosciences).

    Derivative Assay:

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity
    Article Snippet: .. The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec). .. After the incubation with these peptides, the cells were washed once with RPMI and costimulated using purified anti-human CD28 antibody (1 μg/ml) (clone L293; BD Biosciences).

    Positive Control:

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity
    Article Snippet: .. The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec). .. After the incubation with these peptides, the cells were washed once with RPMI and costimulated using purified anti-human CD28 antibody (1 μg/ml) (clone L293; BD Biosciences).



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    CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent <t>donors.</t> <t>PBMCs</t> from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with <t>EBV</t> peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.
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    Image Search Results


    CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent donors. PBMCs from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with EBV peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.

    Journal: Journal of Virology

    Article Title: Identification of SARS-CoV-2 Nucleocapsid and Spike T-Cell Epitopes for Assessing T-Cell Immunity

    doi: 10.1128/JVI.02002-20

    Figure Lengend Snippet: CD8 + T-cell polyfunctionality analysis after ex vivo expansion with specific SARS CoV-2 peptides derived from the SARS-CoV-2 NC. (a and c) Representative dot plots showing effector cytokine production and degranulation level of CD8 + T cells stimulated with RTATKAYNV (a) and IIWVATEGA (c) peptides. The CD8 + T cells were derived from two HLA-A*02-positive SARS-CoV-2 convalescent donors. PBMCs from an HLA-A*02-negative SARS-CoV-2 convalescent donor were used as a negative control. The clinical samples were obtained at 1 to 2 months after SARS-CoV-2 recovery. (b and d) Bar charts represent the effector profiles of RTATKAYNV (b)- and IIWVATEGA (d)-specific CD8 + T cells. PBMCs expanded with EBV peptides were used as positive control. Pies depict the distribution of mono-, bi-, tri-, and tetrafunctional cells within specific CD8 + T cells.

    Article Snippet: The rested PBMCs ([3 to 5] × 10 6 cells) were incubated in the presence of 5 μM of SARS-CoV-2 peptide pools or Epstein-Barr Virus (EBV) peptide mix (MACS GMP PepTivator EBV select, Miltenyi Biotec) for 1 h. Two SARS-CoV-2 peptides derived from the NC ( RTATKAYNV and IIWVATEGA ; 5 μM for each peptide) were used to stimulate PBMCs for 1 h. The EBV peptide mix (resuspended at 100 μg/ml in DMSO) was used for a positive control (MACS GMP PepTivator EBV Select; Miltenyi Biotec).

    Techniques: Ex Vivo, Derivative Assay, Negative Control, Positive Control

    Biological parameters in the 49 SOT patients after stratification by tumor occurrence.

    Journal: Frontiers in Oncology

    Article Title: Biological Predictors of De Novo Tumors in Solid Organ Transplanted Patients During Oncological Surveillance: Potential Role of Circulating TERT mRNA

    doi: 10.3389/fonc.2021.772348

    Figure Lengend Snippet: Biological parameters in the 49 SOT patients after stratification by tumor occurrence.

    Article Snippet: CMV, EBV, Survivin, TERT peptide mixes (ProImmune, Oxford UK; 0.2ng/mL of each peptide mix) or unspecific stimuli (0.5mg/mL αCD3/αCD28) were resuspended in CTL-test Medium, plated in triplicate and incubated for 10-20 minutes.

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    Cox regression analysis evaluating the associations between baseline demographic, clinical or biological parameters and tumor onset.

    Journal: Frontiers in Oncology

    Article Title: Biological Predictors of De Novo Tumors in Solid Organ Transplanted Patients During Oncological Surveillance: Potential Role of Circulating TERT mRNA

    doi: 10.3389/fonc.2021.772348

    Figure Lengend Snippet: Cox regression analysis evaluating the associations between baseline demographic, clinical or biological parameters and tumor onset.

    Article Snippet: CMV, EBV, Survivin, TERT peptide mixes (ProImmune, Oxford UK; 0.2ng/mL of each peptide mix) or unspecific stimuli (0.5mg/mL αCD3/αCD28) were resuspended in CTL-test Medium, plated in triplicate and incubated for 10-20 minutes.

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